Ciap enzyme

What is the unit of CIAP hydrolyzes? Can CIAP be heat inactivated? Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.

This enzyme can be used to reduce linear vector self-ligation and vector background during cloning. M ZnCl and glycerol.

Maya Saleh of the Department of Medicine, have identified an enzyme, cIAPthat helps the lungs protect themselves from the fluby giving them the ability to resist tissue damage. This enzyme is used to prevent recircularization and religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´-termini (1–5). Calf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5´-phosphate groups from DNA , RNA , and ribo- and deoxyribonucleoside triphosphates. CIP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA.

The enzyme acts on 5´ protruding , 5´ recessed and blunt ends. Find out more here. CIAP is frequently used in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes.

Enzymatically mediated bioprecipitation of heavy metals from industrial wastes and single ion solutions by mammalian alkaline phosphatase. In its absence, the same factors that depend on cIAPto keep the cell alive, reveal a destructive side and induce a harmful form of cell death. IAPtherefore not only protects the infected cells from dying in such a manner, but also protects uninfected neighbouring cells in the same tissue. The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of incubation. To narrow down your search, select desired filters or enter keywords in the provided text boxes.

Note the enzyme source, reaction conditions and units of activity provided by the manufacturers before making a purchase. While the digestion of the vector with two different enzymes should prevented ligation of the vector alone, the use of CIAP prevents ligation of any singly cut vector, reducing the number of false positives in the subsequent ligation reactions. An excess of CIAP was used in a 30‐min reaction to treat sufficient linearized vector. SAP is identical to the native enzyme and contains no affinity tags or other modifications. SAP nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters.

We've detected that you are using an older version of Internet Explorer. Unit Definition: One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1μmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10. M 4-nitrophenyl phosphate, 0. M MgCl (pH at 25°C).

The pH optimum is in the alkaline range. Alkaline Phosphatase catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates. A wide variety of enzymes are available for molecular and biochemical applications.

Lysosomal membrane permeabilization is an essential step in TRAIL-induced apoptosis of liver cancer cell lines. TRAIL-induced lysosomal membrane permeabilization is mediated by the multifunctional sorting protein PACS-and repressed by the Eligases cIAP -and cIAP -2. It is routinely used to dephosphorylate proteins and nucleic acids.

This protein is available in the BioUltra Grade. Dilutions can be prepared in 0. M Tris-HCl, mM MgCl pH 8.